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Oral Abstract

Patient Blood Management

Oral Abstract Session - New Insights to Patient Blood Management

#OA2-AM25-ST-22 - Beyond the Operating Room: Preservation of Blood Quality and Erythrocyte Function in Postoperative Salvaged Mediastinal Blood

Saturday, October 25, 2025
1:45 PM - 2:45 PM  PT
Room: SDCC 30AB
Oral Abstracts

    Presenting Author(s)

  • Jiachen Shan, PhD (she/her/hers)

    Resident Physician
    Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
    Beijing, Beijing

    Disclosure information not submitted.

Background/Case Studies: Autologous cell salvage is widely used intraoperatively to reduce allogeneic transfusions. However, the blood quality and erythrocyte function of postoperative salvaged blood remain inadequately characterized, particularly in cardiovascular surgery, postoperative hemorrhage is prevalent. This study evaluated postoperative salvaged blood compared with intraoperative salvaged blood and stored packed red blood cells (PRBC).

Study

Design/Methods:

This in vitro observational study analyzed 180 blood samples (60 per group): postoperative salvaged blood (Post-ACS), intraoperative salvaged blood (ICS), and PRBC. The salvaged samples were processed using a cell salvage system, and PRBC were 15–20 days old. Parameters assessed included hemoglobin, hematocrit, pH, potassium, glucose, lactate, hemolysis rate, erythrocyte deformability, morphology, and intracellular 2,3-diphosphoglycerate (2,3-DPG) and adenosine triphosphate (ATP) levels. ANOVA or Kruskal-Wallis tests were used for group comparisons.

Results/Findings:

All groups met international hemolysis standards (< 0.8%). Post-ACS blood exhibited hemoglobin (17.4±3.1 g/dL) and hematocrit (52.33±9.16%) levels comparable to PRBC, with a near-physiologic pH (7.34±0.05) and significantly lower potassium (1.06±0.38 mmol/L), glucose (0.89[0.82-0.98] mmol/L), and lactate (0.70 [0.30-1.50] mmol/L) concentrations (all p < 0.001). Post-ACS had significantly higher erythrocyte deformability (0.78 [0.70–0.91]), 2,3-DPG (12.21 [7.42-15.65] μmol/g Hb), and ATP (5.50 [4.52-5.92] μmol/g Hb) compared to PRBC (all p < 0.001). Compared to ICS blood, Post-ACS blood had similar metabolic profiles, deformability, and levels of 2,3-DPG and ATP (p > 0.05). Erythrocyte morphology remained biconcave-discoid in all groups.

Conclusions:

Postoperative salvaged blood demonstrated comparable quality and erythrocyte function to intraoperative salvaged blood, with superior metabolic and functional parameters relative to stored PRBC. These findings support its clinical viability as a physiologically favorable autologous transfusion strategy and justify further investigation in large-scale clinical trials.